Genetic diversity of gamma ray aplication result on Mandarin’s SoE using ISSR markers

  • Putri Kendari Universitasy of Lampung
  • Mahfut Mahfut Universitas Lampung
Keywords: Keywords:Mandarin’s SoE, Genetic Variation, ISSR, Mutation breeding.

Abstract

Mandarin’s SoEare one of Indonesia's leading mandarinto substitute imported oranges. The quality of oranges can be improved through breeding programs, one of which is mutation breeding using gamma rays. The purpose of this study was to obtain information about the genetic variation of SoE mandarin resulting from gamma ray radiation using ISSR markers. PCR results with ISSR markers on tangerine plants produced by gamma ray radiation showed various patterns, namely the bands were the same, lost bands, and experienced the addition of new bands compared to control plants.

 

 

References

Doyle, J.J., & Doyle, J.L. (1990). Isolation of Plant DNA From Fresh Tissue. Journal Focus.12(1), pp.13-15.

Farida, Y. et al., (2010). Variation Genetic Oranges Mandarins SoE (Citrus reticulate Blanco) Results of Radiation Rays Gamma Using markers ISSR. Indonesian Citrus and Subtropical Fruit Research Institute. Malang.

Harahap, F. (2005). Induction of genetic variation of mangosteen (Garcinia mangostana L.) by gamma radiation. Dissertation. Postgraduate School, Bogor Agricultural University. Bogor.

Hardiyanto, C. et al., (2007). Diversity analysis of Indonesian mandarin using RAPD primer. Journal of Horticulture. 3, pp.239-246.

Indriati, H. et al., (2016). Evaluation of Diversity of Genetic Mutant Hope Generation MV1 Mandarins SoE (Citrus reticulate Blanco) Be Based Refresh Bookmarks Morphological and ISSR. Journal of Indonesian Hortikultur,7(2), pp.102-110.

Medina, F. et al., (2004). Mutation Breeding Manual. Forum for Nuclear Cooperation in Asia (FNCA). Japan.

Pardal, S. J. (2014). Mutation Techniques for Plant Breeding. In Agricultural Research and Development Articles. Biogen. Jakarta.

Powell, W. et al., (1996). Polymorphism revealed by simple sequence repeats. Trends Plant Genet. 1, pp. 215-222.

Rafalski, J.A. et al., (1996). Generating and using DNA markers in plants. In B. Birren, and E. Lai (eds.) Nonmammalian Genomic Analysis. A Practical Guide. Academic Press. San Diego. pp.75-134.

Sastrosumarjo, S. et al., (2006). Plant Cytogenetics Genetics and Plant Breeding Section. Department of Agronomy and Horticulture. Bogor Agricultural Institute. Bogor.

Shahsavar, A.R. et al., (2007). Characterization of citrus germplasm including unknown variants by inter simple sequence repeat (ISSR) markers. Scientia Horticulturae. 112, pp.310-314.

Tenriulo, A. et al., (2001). DNA Extraction of Kappaphycus alvarezii Marine Grass Clumps Using the Chloroform Phenol Method. Journal. Mar. Chim Acta. 2 (2), pp.6-10.

Van Harten, A. M. (1998). Mutation Breeding: Theory and Practical Applications. Cambridge University Press. Cambridge.

Wardiyati, W. et al., (2002). The effect of cholchisin and gamma ray radiation on the vegetative growth of the moon orchid (Phalaenopsis). Agrivita XXIV. 2, pp. 80-85.

Widiastuti, A. et al., (2013). Analysis of Genetic Diversity of Mangosteen (Garcinia mangostana) Irradiated with Gamma Rays Based on ISSR Markers. Journal Biotechnology. 10(1), pp.15-22.

Published
2021-11-30
Section
Review Article